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XGen proudly offers lipid nanoparticle (LNP) formulation services for mRNA and circular RNA drug research and development service.

 

We are excited to expand our offerings with the introduction of our new Targeted LNP Service. This advanced service is engineered to enhance the precision and efficacy of therapeutic delivery by utilizing state-of-the-art lipid formulations and targeting ligands. Our Targeted LNP Service ensures that therapeutic payloads are delivered directly to specific cells or tissues, thereby maximizing therapeutic impact and minimizing off-target effects.

 

LNPs have been proven to be an effective drug delivery system for RNA therapeutics which can be easily degraded in vivo. The lipid layer can fuse with the cell membrane, allowing the encapsulated RNA to enter the cell and exert its therapeutic effect. They also protect therapeutic agents from degradation and improve their bioavailability LNPs are used in various applications, such as delivering RNA-based therapeutics or small molecules.

 

 

   
Variety of ionizable lipid formulation with high encapsulation efficiency   Variety of ionizable lipid formulation with high encapsulation efficiency   Ready-Edit LNP Formulation
with CRISPR RNA solutions
Details

       

LNP Service Options
 
Characteristics   QC Testing   Payload Requirement   Application
             
  • Ionizable lipid formulations: MC3, SM102, and ALC0315
  • Final concentration:  <2 mg/mL
  • Shipped on dry ice
 
  • Encapsulation efficiency: >85%
  • Polydispersity Index: <0.2
  • Zeta potential: ± 15 mV
 
  • Payload size: 20 nt ~ 20,000 nt
  • Amount: 0.1mg - 1g encapsulated RNA
 
  • Intramuscular injection (I.M.) for animal use only
  • Intravenous injection (I.V.) for animal use only
  • In vitro studies
 
 Manufacturing Workflow
 
Our integrated IVT RNA manufacturing workflow streamlines production from gene synthesis to LNP packaging.
 
 
 
 
Quality Control and Specification
 
Project Detection Testing Standards Standard QC Advanced QC
Appearance Visual observation Clear and free of foreign particles
Final Concentration RiboGreen assay 0.05 - 0.2 mg/mL
Encapsulation Efficiency RiboGreen assay >85%
Encapsulated RNA Integrity Bioanalyzer >75% ×
Partical Size DLS

MC3-LNP   80 - 110 nm

SM102-LNP   65 - 125 nm

Lipid 5-LNP   65 - 125 nm

ALC0315-LNP   50 - 100 nm

PDI DLS <0.2
Zeta Potential DLS ± 15.0 mV ×
pH pH meter 7.4 ± 0.5 ×
Endotoxin LAL endotoxin detection <10 EU/mL ×
Bioburden Pour plate and spread plate assay <10 CFU/100mL ×
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