• Codon optimization for protein expression offered every day for every gene
• Codon optimization with >200 factors screened (patent)

• 100 bp – 20,000 bp gene inserts
• TAT as fast as 6 Bussiness Days to 14 Business Days
• Gene synthesis with Poly(A)-built-in vector

• Ready to use linearized plasmid DNA for IVT
• Vectors encode a stable Poly(A) tail with a variance of ±5–10 nt

• Customized RUO to preclinical grade mRNA service 
• Advanced purification (Oligo dT, dsRNA removal) and a variety of QC options

• Generic ionizable lipid formulation
• Encapsulate 0.1- 30mg of mRNA, siRNA, miRNA,  DNA, and sgRNA delivery
• LNP for gene editing

• 100 bp – 20,000 bp sequences
• 0.1mg to 1g scale

• Cap0
• Cap1 (Co-trascriptional or enzymatic)
• Cap2 (Co-transcriptional)
• Uncapped

• 100 poly(A) tail (recommended)
• Custom tail ≤ 120A

• N1-Me-Ψ
• 5moU
• 5meC &  Ψ
• Cy5-UTP or Thio-CTP
• Unmodified
• 5’-Biotin modified
• N6-methyl-A
• 5-Iodo-C, 5-Iodo-U

• LiCl precipitation
• Oligo dT purification
• dsRNA removal

Quality Control and Specification

Total Protein Residue

We can make mRNA from 100 n.t. to 20,000 n.t. in length. 

What's the difference between RUO and preclinical grade mRNA?

For RUO grade mRNA, we use LiCl precipition method for purification. It is suitable for in vitro and in vivo proof of concept studies.

For preclinical grade mRNA, we use Oligo dT purification to improve RNA purity.

Can XGen provide GMP-grade mRNA?

Yes

Do you guarantee the yield of the mRNA?

Yes.

What concentratio of mRNA can you produce?

We provide mRNA at a concentration of 0.2-2 mg/mL by default. We can adjust the concentration to up to 4mg/mL upon customer's request

What buffer options does XGen offer for mRNA?

1mM sodium citrate (pH 6.5)

TE buffer (pH7)

RNase-Free water.